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KMID : 0855520070200020093
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Korean Journal of Physical Anthropology
2007 Volume.20 No. 2 p.93 ~ p.104
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Changes of Immunoreactivities of Calcium Channel α1B Subunit in Myenteric Plexus of Capsaicin Treated Adult Rats
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Park Seung-Hwa
Kim Sang-Hyun
Kim Kyung-Rae
Park Ung-Chae
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Abstract
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This study was performed to investigate the changes of immunoreactivities of calcium channel α1B subunit in myenteric plexus of capsaicin treated adult rats.
Sprague Dawley rats (about 200 g) were injected with capsaicin (50 ㎎/㎏) subcutaneously. Ileal myenteric plexus was prepared by whole mount preparation method in 1 day, 2 days, 1 week and 4 weeks after capsaicin treated adult rats. Each myenteric plexus was reacted with NADH-TR and immunostained with α1B subunit. Myenteric ganglion cell numbers were counted by image analyzer.
In control group myenteric plexus was arranged in rectangular appearance; myenteric ganglia and internodal strand were immunoreacted with α1B subunit. Immunoreactive cells were 56.0% of total myenteric neurons. Total numbers of immunoreactive cells decreased by 19.0%, 37.9% and 64.9% in 1 day, 2 days, and 1 week after capsaicn treated group respectively. In 4 weeks after capsaicin treated group, immunoreactivities of α1B subunit were increased compared to that of the 1 week after group. However total numbers of immuoreactive cells decreased by 43.9% compared to that of the control group.
In conclusion, we confirmed that immunoreactivities of α1B subunit were decreased until 1 week after capsaicin treatment and recovered progressively after that time. It will take more than 4 weeks latency to recover the control immunoreactivities of α1B subunit.
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KEYWORD
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capsaicin, Adult rat, Myenteric plexus, Calcium channel α1B subunit, Imunohistochemistry
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